Press Release (ePRNews.com) - GöTTINGEN, Germany - Feb 02, 2016 - Zika virus is enveloped and icosahedral with a non-segmented, single-stranded, positive sense RNA genome. It is most closely related to the Spondweni virus and is one of the two viruses in the Spondweni virus clade. The virus was first isolated in 1947 from a rhesus monkey in the Zika Forest of Uganda, Africa, and was isolated for the first time from humans in 1968 in Nigeria. Common symptoms of infection with the virus include mild headaches, maculopapular rash, fever, malaise, conjunctivitis, and arthralgia. In 2009, it was proved that Zika virus can be sexually transmitted between humans.
The Zika Virus (ZIKV) real time RT-PCR Kit contains a specific ready-to-use system for the detection of the Zika Virus using RT-PCR (Reverse Transcription Polymerase Chain Reaction) in the real-time PCR system. The master contains a Super Mix for the specific amplification of the Zika Virus RNA.
The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT), during which the Zika Virus RNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by means of PCR (polymerase chain reaction).
Fluorescence is emitted and measured by the real time systems´ optical unit during the PCR. The detection of amplified Zika Virus DNA fragment is performed in fluorimeter channel FAM with the fluorescent quencher BHQ1. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.
For use with ABI Prism®7000/7300/7500/7900/Step One Plus; iCycler iQ™4/iQ™5; Smart Cycler II;Bio-Rad CFX 96;Rotor Gene™6000; Mx3000P/3005P;MJ-Option2/Chromo4; LightCycler®480 Instrument
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MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.
In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.